| 摘要 |
Provided is a method for determining positions of 5-hydroxymethylated CG sites in DNA, which method comprises a step of contacting the nucleic acid sequence with DNA methyltransferase and a selenol compound under conditions which allow the DNA methyltransferase to attach the selenol to said 5-hydroxymethylcytosine residues and then produce covalent inter-nucleotide cross-links, which lead to DNA strand scission at said positions upon action of piperidine. |
