METHOD FOR ANTIGEN AND ANTIBODY DETERMINATION IN BLOODGROUP SEROLOGY

摘要

Method and test kit for assaying in a sample an analyte which is a bloodgroup antigen present on erythrocytes or an antibody binding to such a bloodgroup antigen. To that end, the sample is treated with a reagent containing a binding partner for the analyte, so that a complex of bloodgroup antigen present on erythrocytes and antibody bound thereto is formed if the sample contains analyte. It the analyte is a bloodgroup antigen present on erythrocytes, the analyte binding partner is an antibody capable of binding to the bloodgroup antigen and if the analyte is an antibody binding to a bloodgroup antigen, the analyte binding partner is the bloodgroup antigen present on erythrocytes. Erythrocytes, complexed or non-complexed, are then separated from non-bound antibodies using a separation medium with a density higher than that of the liquid containing the antibodies but lower than the density of erythrocytes. Complexed erythrocytes are separated from non-complexed erythrocytes by binding complexed erythrocytes to a solid surface on which an immunoglobulin-binding substance is immobilized and discharging non-complexed erythrocytes to a deepened zone of the reaction vessel. The result is then determined by detecting where the erythrocytes are located.