ISOLATED DNA FRAGMENT, A METHOD OF PREPARING A RECOMBINANT PROTEIN EXHIBITING NEUROTROPIC ACTIVITY, PROTEIN NT-3 AND A PHARMACEUTICAL COMPOSITION

摘要

FIELD: biotechnology, genetic engineering, pharmacy. SUBSTANCE: invention relates to human protein neurotropin-3 (NT-3) by culturing mammalian cells transformed by an expression vector that contains the isolated DNA fragment encoding the indicated protein. Isolated DNA fragment involves a nucleotide sequence that encodes a precursor of human neurotropin-3 consisting of an amino acid sequence splitting by protease and the following amino acid sequence of matured protein neurotropin-3. Protein NT-3 has a molecular mass 14 kDa, it is able to initiate survival, growth or differentiation of neurons, intergrowing nodular and dorsal ganglions from radices. A pharmaceutical composition is obtained that has protein NT-3 as an active component taken at therapeutically effective dose and a pharmaceutically acceptable carrier. Obtained protein NT-3 can be used for diagnosis and treatment of patients with different neurological disorders. EFFECT: improved of protein preparing. 5 cl, 45 dwg, 6 tbl, 5 ex