High efficiency methods of sex sorting sperm

摘要

A method of efficiently sorting a sperm sample in a particle sorting instrument comprises a) establishing a sheath fluid stream in the particle sorting instrument; b) flowing a sperm sample into the sheath fluid stream; c) identifying viable X-chromosome bearing sperm and/or viable Y-chromosome bearing sperm in the sperm sample; d) separating the viable X-chromosome bearing sperm and/or the viable Y-chromosome bearing sperm from the remainder of the sperm sample; e) collecting the viable X-chromosome bearing sperm and/or the viable Y-chromosome bearing sperm; f) determining one or more measured sorting parameters in the particle sorting instrument; g) establishing a minimum productivity threshold; h) establishing a minimum purity threshold; i) determining a sorting efficiency based on the measured sorting parameters determined while sorting; and j ) adjusting one or more instrument parameters to increase the sorting efficiency while maintaining the productivity above the minimum productivity threshold and purity above the minimum purity threshold.