Method and system for analyzing a colorimetric assay

摘要

Described herein is a method, system and computer program for analyzing a colorimetric assay that includes obtaining an image of the assay, optionally correcting for ambient lighting conditions in the image, converting the intensity data for at least one of the red channel, the green channel, or the blue channel to a first data point, recalling a predetermined standardized curve, comparing the first data point with the standardized curve, and identifying the value for the assay parameter from the standardized curve.

主权项

1. A method of analyzing a colorimetric assay to identify a value for an assay parameter, the method comprising: obtaining an image of a first colorimetric assay; converting intensity data from at least one of the red channel, the green channel, or the blue channels from at least a portion of the image of the first colorimetric assay to a first data point having a first value and a second value wherein the first value and the second value indicate the chromaticity of the test colorimetric assay; recalling a predetermined standardized curve from a storage medium, the standardized curve including a plurality of data points, each of the plurality of data points having a third value, a fourth value, and a fifth value, the third value and fourth values indicating the chromaticity of the data point along the standardized curve and the fifth value indicating the value of an assay parameter associated with the third value and the fourth value; and comparing the first data point with the standardized curve to identify the value of the assay parameter for the first colorimetric assay, wherein the image is obtained with a device having an imaging sensor selected from the group consisting of a charge-coupled device (“CCD”) sensor, a complementary metal-oxide-semiconductor (“CMOS”) sensor, and a contact image sensor (“CIS”).