STRAIN OF CULTURED MURINE HYBRIDOMA CELLS USED FOR PREPARING MONOCLONAL ANTIBODIES RAISED TO NUCLEOLUS ANTIGEN ASSOCIATED WITH CELLULAR PROLIFERATION

摘要

FIELD: biotechnology, medicine. SUBSTANCE: hybridoma is obtained by fusion of murine splenocytes immunized with nuclear fraction from cells of beta-lymphoblastoid strain Daudi with murine myeloma cells. Hybridoma produces monoclonal antibodies directed to nucleolus antigen (molecular mass is 35 and 43 kDa) that is detected in proliferating cells of different species, tissue and strain belonging. Invention ensures to study the process of cellular proliferation in malignant diseases and can be used for identification of proliferating cells. EFFECT: strain indicated above, improved method of hybridoma preparing.