Reducing adapter dimer formation

摘要

Provided herein is a method of reducing adapter dimer formation comprising contacting a sample comprising target nucleic acid sequences with 5′ and 3′ adapters in the presence of one or more hairpin oligonucleotides. Also provided is a method of preparing a library of nucleic acid sequences comprising contacting first adapter oligonucleotides with a sample comprising target nucleic acid sequences under conditions to form first ligation products, contacting the sample with one or more hairpin oligonucleotides that binds to the first adapter oligonucleotides, and contacting the sample with second adapter oligonucleotides under conditions to bind to the first ligation products and form second ligation products, wherein the second ligation products form the library of nucleic acid sequences.

主权项

1. A method of reducing adapter dimer formation comprising:
(a) contacting first adapter oligonucleotides with a sample comprising target RNA sequences under conditions to form first ligation products; (b) after step (a), contacting the sample with one or more hairpin oligonucleotides having a 5′ overhang portion configured to bind unligated first adapter oligonucleotides whereby said 5′ overhang portion is complementary to and anneals to at least a portion of said unligated first adapter oligonucleotides; (c) after step (b), contacting the sample with second adapter oligonucleotides under conditions to bind to the first ligation products and form second ligation products, wherein the second ligation products form the library of nucleic acid sequences, thereby reducing adapter dimer formation.