PKD constructs and screening methods

摘要

The invention provides an isolated nucleic acid molecule comprising the complete open reading frame of the C. elegans pkd-2 gene. The invention further provides assay methods for use in the identification of compounds which affect the activity of PKD2 and genetic suppressors of pkd-2, which methods are based on correction of an altered mating phenotype observed in male C. elegans which either overexpress the PKD2 protein or carry a deletion mutation in the pkd-2 gene.