| 摘要 |
The present invention provides a novel method of genomic analysis. In the method, an extension primer generated by capturing an unknown DNA sequence with an adaptor containing a class IIS RE recognition site is used in an extension/amplification reaction with a DNA sample to amplify a unique product containing the captured sequence. The method of the invention therefore allows identification and analysis of unknown sequences upstream to a known region or a sequence. The invention has a particular application to genotyping, including bacterial strain typing. Specifically, the invention provides a method of preparing extension primers to capture sequences from different genomic locations and their use as genetic markers. The invention also provides adaptors to prepare extension primers. |
