METHODS AND COMPOSITIONS FOR TARGETED NUCLEIC ACID SEQUENCE COVERAGE

摘要

The present invention is directed to methods, compositions and systems for analyzing sequence information from targeted regions of a genome. Such targeted regions may include regions of the genome that are poorly characterized, highly polymorphic, or divergent from reference genome sequences.

主权项

1. A method for sequencing one or more selected portions of a genome, the method comprising:
(a) providing starting genomic material; (b) distributing individual nucleic acid molecules from the starting genomic material into discrete partitions such that each discrete partition contains an individual nucleic acid molecule; (c) amplifying selected portions of at least some of the individual nucleic acid molecules in the discrete partitions to form a population of amplicons; (d) barcoding the population of amplicons to form a plurality of barcoded fragments of the amplicons, wherein fragments within a given discrete partition each comprise a common barcode, thereby associating each fragment with the individual nucleic acid molecule from which it is derived; (e) obtaining sequence information from the plurality of fragments thereby sequencing one or more selected portions of a genome.