发明名称 |
Highly expressible genes |
摘要 |
The present invention provides methods of producing protein in a recombinant expression system that comprises translation of mRNA transcribed from a heterologous DNA sequence in the expression system, said method comprising the steps of predicting the secondary structure of mRNA transcribed from a native heterologous DNA sequence; modifying the native heterologous DNA sequence to produce a modified heterologous DNA sequence wherein mRNA transcribed from the modified heterologous DNA sequence has a secondary structure having increased free energy compared to that of the secondary structure of the mRNA transcribed from the native heterologous DNA sequence; and using the modified heterologous DNA sequence in the recombinant expression system for protein production. The invention also provides injectable pharmaceutical compositions comprising a nucleic acid molecule that includes a modified coding sequence. The invention also provides recombinant viral vectors comprising a nucleic acid molecule that includes a modified coding sequence.
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申请公布号 |
US2002123099(A1) |
申请公布日期 |
2002.09.05 |
申请号 |
US20010971806 |
申请日期 |
2001.10.04 |
申请人 |
WEINER DAVID B.;YANG JOO-SUNG |
发明人 |
WEINER DAVID B.;YANG JOO-SUNG |
分类号 |
C12N15/09;A61K35/76;A61K38/00;A61K38/16;A61K38/22;A61K38/43;A61K39/00;A61K39/12;A61K48/00;A61P1/04;A61P3/10;A61P5/14;A61P9/00;A61P17/00;A61P17/06;A61P19/02;A61P21/00;A61P25/00;A61P35/00;A61P37/02;A61P43/00;C07K14/16;C07K14/18;C12N15/67;C12P21/02;C12Q1/02;G01N33/15;G01N33/50;G01N33/53;G01N33/569;(IPC1-7):C12P21/02;G06F19/00;G01N33/48;C12P21/06 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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