发明名称 |
Fluorescent protein sensors for detection of analytes |
摘要 |
Fluorescent indicators including a binding protein moiety, a donor fluorescent protein moiety, and an acceptor fluorescent protein moiety are described. The binding protein moiety has an analyte-binding region which binds an analyte and causes the indicator to change conformation upon exposure to the analyte. The donor moiety and the acceptor moiety change position relative to each other when the analyte binds to the analyte-binding region. The donor moiety and the acceptor moiety exhibit fluorescence resonance energy transfer when the donor moiety is excited and the distance between the donor moiety and the acceptor moiety is small. The indicators can be used to measure analyte concentrations in samples, such as calcium ion concentrations in cells.
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申请公布号 |
US2002165364(A1) |
申请公布日期 |
2002.11.07 |
申请号 |
US20000554000 |
申请日期 |
2000.04.20 |
申请人 |
TSIEN ROGER Y.;MIYAWAKI ATSUSHI |
发明人 |
TSIEN ROGER Y.;MIYAWAKI ATSUSHI |
分类号 |
C07K14/435;G01N33/542;(IPC1-7):C12P21/06;C12P21/04;C07K2/00;C07K4/00;C07K5/00;C07K7/00;C07K14/00;C07K16/00;C07K17/00;A61K38/00;C07K1/00;C07K17/14;C12P21/08 |
主分类号 |
C07K14/435 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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