METHOD FOR SIMULTANEOUS ANALYSIS FOR 18 COMPONENTS IN BILE ACID

摘要

PROBLEM TO BE SOLVED: To provide a stable method for separation-determining highly sensitively a multi-component bile acid such as bile acid in a human serum directly and easily in a short time without requiring a technique such as solid phase extraction. SOLUTION: In this simultaneous analytical method for the multi-component bile acid by an LC-MS method, respective bile acid components are separation- determined by changing continuously and/or stepwisely a composition of a mobile phase from (i) at least an initial composition A of an aqueous solution of pH 9-11 containing 10-25 wt.% of water-soluble organic solvent and 0.05-0.2 M of salt to (ii) at least an initial composition B of an aqueous solution of pH 9-11 containing 35-50 wt.% of water-soluble organic solvent and 0.05-0.2 M of salt, using gradient elution by two liquid or more.