BASE SEQUENCE-DETERMINING APPARATUS AND BASE SEQUENCE- DETERMINING METHOD

摘要

PROBLEM TO BE SOLVED: To provide a base sequence-determining apparatus using a confocal microscope. SOLUTION: This base sequence-determining apparatus using as an analysis sample a carrier on which a nucleic acid labeled with fluorescent substances in a state that the kinds of the fluorescent substances correspond to the kinds of bases comprises a measurement cell having a flow channel therein; a means for transporting a suspension of the carrier to which the nucleic acid of the analysis sample is adhered; a first laser light source and the first optical system for catching the carrier; a means for adding in the flow channel an enzyme solution for sequentially cleaving off mononucletides constituting the nucleic acid from the terminal; a second laser light source and the second optical system for exciting in the cleavage order the fluorescent substances labeling the mononucleotide bases cleaved off with the enzyme solution; the third optical system for sequentially focusing fluorescent lights from the fluorescent substances; a light detector for sequentially detecting the focused fluorescent lights; and an analysis means for reading the base sequence of the nucleic acid from the identification signals outputted from the light detector. COPYRIGHT: (C)2003,JPO