Multiplexed digital assay with data exclusion for calculation of target levels

摘要

Digital assay system, including methods and apparatus, for calculating a level of one or more targets. In an exemplary method, data for amplification of a plurality of targets including a first target may be collected from partitions. A level of the first target may be calculated from only a subset of the data that excludes partitions according to target content.

主权项

1. A method of performing a multiplexed digital assay, the method comprising:
providing a mixture including a plurality of targets and reagents sufficient for amplification of each of the targets, wherein the plurality of targets includes a first target; forming partitions each including a portion of the mixture; amplifying the plurality of targets in the partitions; collecting data from a plurality of the partitions for amplification of each of the plurality of targets; identifying partition populations in the data, each partition population having a different target content of the plurality of targets; and calculating with a processor a level of the first target from only a portion of the data, wherein the portion of the data is collected from only a subset of the plurality of the partitions, wherein the subset of partitions excludes two or more of the partition populations, and wherein the two or more excluded partition populations include at least a pair of excluded partition populations that are not resolved from each other in the data.