发明名称 |
Mutant kanamycin nucleotidyltransferases from S. aureus |
摘要 |
It is desirable to have selective markers suitable for screening of thermophilic bacteria such as Thermus thermophilus. T. thermophilus are good research materials for investigating the interrelation between enzyme structures and functions since they are stable at extreme pH, crystallize easily and are easy-to-handle. Novel mutants of Staphylococcus aureus kanamydn nucleotidyltransferase with markedly improved thermostability are disclosed, as well as a selective marker using the same, and a screening method for thermophilic bacteria such as T. thermophilus using said selective marker.
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申请公布号 |
US6723543(B1) |
申请公布日期 |
2004.04.20 |
申请号 |
US20000697186 |
申请日期 |
2000.10.27 |
申请人 |
RIKEN |
发明人 |
YOKOYAMA SIGEYUKI;HOSEKI JUN;YANO TAKATO;KOYAMA YOSHINORI;KURAMITSU SEIKI;KAGAMIYAMA HIROYUKI |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/10;C12N9/12;C12Q1/48;C12R1/01;C12R1/19;(IPC1-7):C12N9/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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