Amplifying immunodetection of pathological prion protein, useful for diagnosis of spongiform encephalopathy, by adding a macrocyclic adjuvant ligand before reaction with antibody

摘要

Amplifying the immunodetection of pathological prion protein (PrPsc) comprising adding to the test sample a macrocyclic adjuvant ligand (MAL); treatment with protease K, then contacting the treated sample with anti-PrPsc antibodies (Ab). An Independent claim is also included for a kit for diagnosing PrPsc-related diseases, or for immunodetection of PrPsc, that contains MAL.