Automated epifluorescence microscopy for detection of bacterial contamination in platelets

摘要

A method for determining the presence of bacteria in a platelet or red blood cell sample is disclosed. The method includes the steps of: lysing a substantial portion of the platelets or red blood cells; staining the bacteria using a membrane permeable nucleic acid stain; filtering the sample using a membrane filter with a suitable pore size so that a material containing the stained bacteria is retained on the membrane filter; and analyzing the material retained on the membrane filter using epifluorescence microscopy and/or digital image acquisition and analysis to determine the presence of bacteria in the sample. The method allows the detection of bacterial contamination in platelets or red blood cells at clinically significant levels in a relatively short period of time.