发明名称 |
Site-specific GLP-2 conjugate using an immunoglobulin fragment |
摘要 |
Provided are a glucagon-like peptide-2 (GLP-2) conjugate containing native GLP-2 or its derivative and an immunoglobulin Fc fragment being covalently linked via a non-peptidyl polymer, wherein the native GLP-2 or its derivative has a thiol group introduced at its C-terminal end, and one end of the non-peptidyl polymer is linked to an amino acid residue of the GLP-2 other than the N-terminal amino group thereof; a method for preparing the GLP-2 conjugate; a pharmaceutical composition comprising the same; and a method for treating or preventing intestinal disease, intestinal injury, or gastrosis by using the same. Since the GLP-2 conjugate of the present invention has a remarkably increased binding affinity to a GLP-2 receptor, it shows a prolonged in vivo half-life and an improved in vivo durability and stability. |
申请公布号 |
US9504757(B2) |
申请公布日期 |
2016.11.29 |
申请号 |
US201214369436 |
申请日期 |
2012.12.28 |
申请人 |
HANMI SCIENCE CO., LTD. |
发明人 |
Kim Seung Su;Lim Se Young;Jung Sung Youb;Kwon Se Chang |
分类号 |
A61K38/26;A61K47/48 |
主分类号 |
A61K38/26 |
代理机构 |
Sughrue Mion, PLLC |
代理人 |
Sughrue Mion, PLLC |
主权项 |
1. A glucagon-like peptide-2 (GLP-2) conjugate comprising native GLP-2 or its derivative and an immunoglobulin Fc fragment being covalently linked via a non-peptidyl polymer, wherein the native GLP-2 or its derivative has a thiol group introduced at its C-terminal end, and one end of the non-peptidyl polymer is linked to the C-terminal thiol group of the GLP-2 or its derivative,
wherein the derivative of GLP-2 is selected from the group consisting of a GLP-2 derivative prepared by removing α-carbon of histidine which is the first amino acid at the N-terminal end of the native GLP-2 and an N-terminal amine group bound thereto; a GLP-2 derivative prepared by deleting an N-terminal amine group of the native GLP-2; a GLP-2 derivative prepared by substituting an N-terminal amine group of the native GLP-2 with a hydroxyl group; a GLP-2 derivative prepared by modifying an N-terminal amine group of the native GLP-2 with a dimethyl group; and a GLP-2 derivative prepared by substituting an N-terminal amine group of the native GLP-2 with a carboxyl group. |
地址 |
Hwaseong-Si KR |