Method and device for the concentration of multiple microorganisms and toxins from large liquid toxins

摘要

A method for the simultaneous concentration of multiple toxins from large volumes of water. The method includes the steps of providing a disposable separation centrifuge bowl, the centrifuge bowl including a positively charged material at it's inner core. A large water sample contaminated with toxins from a group consisting of protozoa, bacteria, bacterial spores, and toxins is delivered to the centrifuge bowl. A centrifugal force is applied to the separation bowl. The water sample is concentrated to remove large particles of the toxins in the bowl due to the centrifugal forces. The concentrated water sample is passes through the positively charged inner core to capture any remaining concentrated targets by electrostatic forces and the concentrated targets are eluted.

主权项

1. A device for concentrating multiple pathogens from large liquid samples comprising:
a centrifuge bowl; a core located within the centrifuge bowl, the core comprising an outer core and an inner core, the outer core and the inner core comprising a core material having a plurality of holes in the core material; a layer of positively charged filter material located between the outer core and the inner core; an inner fluid passageway located within the core; and an inlet located at one end of the centrifuge bowl of receiving the liquid sample containing the pathogens and delivering the liquid sample to the core, and wherein the plurality of hole in the core material allows the liquid sample to flow through the core material to the positively charged material such the concentrated pathogens are attracted and adhere to the positively charge material.