Interlacing to improve sampling of data when ramping parameters

摘要

Systems and methods are provided for interlacing ramped mass spectrometer parameter values during data acquisition. Ions from a sample are acquired within a cycle time, Ct, using a mass spectrometer. Within each Ct, two or more scans of the acquired ions are performed using two or more ramped values for a parameter of the mass spectrometer. When it is determined that scans for a desired range of ramped parameter values cannot be performed within Ct, the desired range of ramped values is divided into at least two interlaced groups of ramped values. The mass spectrometer is instructed to perform scans for each of the interlaced groups within two or more cycle times. Spectra from the scans for each of the at least two interlaced groups are combined. The ramped parameter values of the combined spectra have the desired range and the desired effective step size.

主权项

1. A system for interlacing ramped mass spectrometer parameter values during data acquisition in order to maintain a desired range and step size for the values, comprising:
a mass spectrometer that repeatedly acquires ions from a sample within a cycle time, Ct, and within each Ct performs two or more scans of the acquired ions using two or more ramped values for a parameter of the mass spectrometer, wherein each of the two or more scans occurs within a step time, St, and each of the two or more ramped values vary by a step size, Ss, and a processor in communication with the mass spectrometer that
calculates if scans for a desired range of ramped values for the parameter can be performed within Ct using the desired range of ramped values, St, and Ss, andif the scans for the desired range cannot be performed within Ct,
divides the desired range of ramped values into at least two interlaced groups of ramped values, wherein each of the ramped values in each of the at least two interlaced groups of ramped values vary by a step size of at least 2Ss,instructs the mass spectrometer to perform scans for each of the at least two interlaced groups within two or more separate cycle times, andcombines spectra from the scans for each of the at least two interlaced groups producing combined spectra for the two or more separate cycle times that span the desired range and have an effective step size of Ss across the desired range.