Method for calibrating clinical chemistry instruments

摘要

A method of deriving correction for instrument-to-instrument variations in the illumination band centroid wavelengths and wavelength band shapes of the optical systems of clinical chemistry instruments includes the steps of determining the centroid wavelength and wavelength band shape of a light source used in the optical system of a clinical chemistry instrument to provide a determined wavelength band shape and centroid wavelength, comparing the determined wavelength band shape and centroid wavelength with a known reflection density or absorbance wavelength spectrum of a specific type of chemical reagent test to provide a correction value, and calculating the correction value, which is to be used to modify a reflection density or absorbance measurement taken by the instrument of a reagent test of the a specific type of chemical reagent test.

主权项

1. A method of calibrating an optical system of a dry chemistry analyzer, the method comprising the steps of:
causing at least one light emitting diode of the optical system to emit light, the light emitted by the at least one light emitting diode of the optical system of the dry chemistry analyzer having a wavelength band shape and centroid wavelength, the at least one light emitting diode being provided to illuminate a chemical reagent test slide, the reagent test slide having a reflection density spectrum associated therewith, the reagent test slide, when illuminated by the at least one light emitting diode, reflecting light, the optical system of the dry chemistry analyzer having a light detector, the light detector receiving light reflected from the reagent test slide illuminated by the light from the at least one light emitting diode, the light detector providing a signal in response to the reflected light received by the light detector; in an off-line test performed prior to the complete assembly of the dry chemistry analyzer, measuring the wavelength band shape and centroid wavelength of non-reflected light directly emitted by the at least one light emitting diode to provide a measured wavelength band shape and centroid wavelength; comparing the measured wavelength band shape and centroid wavelength with a reference wavelength band shape and centroid wavelength; and calculating one or more correction values, the one or more correction values providing a correction which is used to modify the reflection density determined from the signal provided by the light detector.