A BIOREACTOR SYSTEM AND METHOD FOR PRODUCING A BIOPOL YMER

摘要

Provided is a bioreactor system and a fermentation process employing continuous inline medium dilution in which concentrated medium and nutrients are blended with water or buffer and fed to the cell culture vessel of a bioreactor system, e.g. for production of antibodies and other recombinant proteins by mammalian cells.

主权项

1. A bioreactor system for producing a product selected from a cell and a biopolymer expressed by a cell, wherein the bioreactor system is a continuous production system and wherein the bioreactor system comprises:
a cell culture vessel (5) comprising a product harvest module (6); optionally, a bleed outlet (9); a medium container (1;2); a water and/or buffer supply (3); and an inline medium dilution system (4) for diluting concentrated medium from the medium container, the inline medium dilution system having an inlet and an outlet, wherein the inlet is in fluid communication with the medium container and the water/buffer supply, and wherein the outlet is in fluid communication with the culture vessel.