High throughput selection of specific cell binding and lytic polypeptides

摘要

The invention provides methods for identifying cell binding and/or lytic polypeptides that permit production of specific polypeptide therapeutics in a high throughput manner.

主权项

1. A method for identifying lytic polypeptides, comprising
(a) contacting one or more addressable polypeptide arrays with:
(i) a first population of cells of interest comprising an intracellular detectable marker (IDM); and(ii) a second population of the cells of interest comprising a cell surface detectable marker (CSDM);wherein the addressable polypeptide arrays comprise at least 1000 polypeptides of between 15-24 amino acids in length in defined locations on the array(s), and wherein the contacting occurs under conditions suitable to promote binding of polypeptides to the cells of interest; and (b) detecting binding of the polypeptides to the cells of interest; wherein a polypeptide that binds cells in the first population of cells without damage to the cells and binds cells in the second population of cells without damage to the cells is a binding polypeptide for the cells of interest; and wherein a polypeptide that binds cells in the first population of cells with damage to the cells and binds cells in the second population of cells with damage to the cells is a lytic polypeptide for the cell of interest.