| 摘要 |
<p>The method of this invention is characterized by the use of hydrolyzed cross-linked polyacrylamide particles to which have been bonded, by means of covalent bonds, antibodies against the thyroid hormone to be determined. The particles selected are of a size which forms a stable hydrophyllic suspension. In the preferred embodiment a measured quantity of unextracted human serum is mixed together with a blocking agent in an amount sufficient to displace the thyroid hormone to be measured from thyroxine binding globulin (TBG), a radioactively labeled thyroid hormone of the type to be measured, and the antibody-pol-yacrylamide complex. The hormone to be measured is displaced by the blocking agent followed by the competitive binding of the labeled and unlabeled hormone to the antibody-polyacrylamide particles. The particles are readily separated from the sample liquid and the radioactivity of the particle material and/or in the liquid is determined. Particles containing Alcian yellow or blue dye facilitate practice of the method.</p> |
