DETECTING SINGLE NUCLEOTIDE POLYMORPHISM USING OVERLAPPING HYDROLYSIS PROBES

摘要

Methods for the rapid detection of the presence or absence of a SNP in a target nucleic acid in a sample are described. The methods can include performing an amplifying step, a hybridizing step utilizing a double stranded probe with two overlapping SNP specific hydrolysis probe sequences where one of the probe sequences can include a hairpin structure toward the 3′ end, and a detecting step. Furthermore, the double stranded SNP specific hydrolysis probes along with kits are provided that are designed for the detection of a SNP in a target nucleic acid.

主权项

1. A kit for detecting a SNP in a target nucleic acid in a sample, comprising:
a first primer comprising a first nucleic acid sequence and a second primer comprising a second nucleic acid sequence specific to produce an amplification product comprising a sense strand and an anti-sense strand of the target nucleic acid; and a double stranded probe comprising:
a first SNP specific hydrolysis probe comprising a third nucleic acid sequence complementary to a region of the sense strand that contains the SNP, the first SNP specific hydrolysis probe comprising a first interactive label and a second interactive label, a first 5′ end and a first 3′ end; anda second SNP specific hydrolysis probe comprising a fourth nucleic acid sequence complementary to a region of the anti-sense strand that contains the SNP, the second SNP specific hydrolysis probe comprising a third interactive label and a fourth interactive label, a second 5′ end and a second 3′ end.