| 摘要 |
PURPOSE:The titled preparation that is made by confining urokinase in spaces in fine lipid particles, thus removing bad influences caused by parenteral administration and making it possible to keep prolonged activity in blood. CONSTITUTION:A solution of a lipid, e.g., phospholipid such as lecithin or phosphatidylcholine, in a solvent that causes no denaturation of the lipid such as ethanol is treated to remove the solvent. Then, the lipid is made into films. At this time, cholesterol or phosphatidic acid may be added as a stabilizer. Another solution of urokinase in a solvent that causes no denaturation of the lipid such as a phosphoric acid buffer solution of 6-8pH is added to the film and they are vigorously stirred. Preferably, they are treated with ultrasonic waves to disperse the film uniformly, resulting in the inclusion of urokinase in spaces of the lipid. The addition of a high- molecular stabilizer such as albumin gives the preparation with highly increased stability. |
