DETECTION, ISOLATION AND IDENTIFICATION OF MICROORGANISMS

摘要

A method of selectively identifying live microorganisms in a sample involves contacting a sample suspected of containing a live microorganism of interest with a biomolecular precursor labeled with a reactive chemical label. The live microorganism of interest is grown under conditions that promote selective growth of the microorganism to permit the microorganism to utilize the labeled biomolecular precursor to synthesize labeled biomolecules on a cell surface of the live microorganism. Labeled live microorganisms are contacted with a reporter and/or capture element bearing a functional group that reacts with the reactive chemical label. The labeled live microorganism is analyzed to identify it. The method can specifically isolate live microorganisms from dead ones thereby reducing the possibility of false identification of contaminating microorganisms and is able to detect specific strains using tailored metabolites that are specific for a given microorganism. The present method is considerably faster than currently used cell culture-based methods.

主权项

1. A method of selectively identifying live microorganisms in a soil, food or water sample comprising:
a) contacting a sample suspected of containing a live microorganism of interest with a biomolecular precursor labeled with a reactive chemical label; b) growing the live microorganism of interest under conditions that promote selective growth of the microorganism to permit the microorganism to utilize the labeled biomolecular precursor to synthesize labeled biomolecules on a cell surface of the live microorganism to produce a labeled live microorganism; c) contacting the labeled live microorganism with a reporter and/or capture element bearing a functional group that reacts with the reactive chemical label to permit identification of the live microorganism; and, d) analyzing the labeled live microorganism to identify it.