| 发明名称 |
METHOD FOR TRANSFORMATION OF STRAMENOPILE |
| 摘要 |
To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid. |
| 申请公布号 |
US2016289689(A1) |
申请公布日期 |
2016.10.06 |
| 申请号 |
US201514711075 |
申请日期 |
2015.05.13 |
| 申请人 |
KYUSHU UNIVERSITY, NAT'L UNIVERSITY CORPORATION ;UNIVERSITY OF MIYAZAKI ;KONAN GAKUEN ;NIPPON SUISAN KAISHA, LTD. |
发明人 |
Sakaguchi Keishi;Hamaguchi Rie;Matsuda Takanori;Ito Makoto;Nagano Naoki;Hayashi Masahiro;Honda Daisuke;Okita Yuji;Sugimoto Shinichi |
| 分类号 |
C12N15/79;C12N9/02;C12N15/113;C12N9/10;C12P7/64;C12N15/89 |
主分类号 |
C12N15/79 |
| 代理机构 |
|
代理人 |
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| 主权项 |
1. A method for transforming Stramenopile, the method comprising disrupting a stramenopile gene and/or inhibiting expression thereof by genetic engineering in microorganisms belong to Stramenopile, wherein the microorganisms are selected from the group consisting of Thraustochytrium aureum ATCC 34304 and Thraustochytrium roseum ATCC 28210. |
| 地址 |
Fukuoka-shi JP |
