| 发明名称 |
METHOD FOR MANUFACTURING TELENCEPHALON OR PROGENITOR TISSUE THEREOF |
| 摘要 |
The present invention provides a method of producing more mature telencephalon or a progenitor tissue thereof, in vitro, from mammalian pluripotent stem cells, comprising obtaining a telencephalon marker-positive aggregate by culturing an aggregate of pluripotent stem cells in suspension in the presence of a Wnt signal inhibitor and a TGFβ signal inhibitor, and further culturing the telencephalon marker-positive aggregate in suspension under a high oxygen partial pressure condition. In one embodiment, the suspension culture under a high oxygen partial pressure condition is performed in the presence of a Wnt signal enhancer and a bone morphogenetic factor signal transduction pathway activating substance. |
| 申请公布号 |
US2016289635(A1) |
申请公布日期 |
2016.10.06 |
| 申请号 |
US201415037926 |
申请日期 |
2014.11.21 |
| 申请人 |
RIKEN |
发明人 |
SASAI Yoshiki;KADOSHIMA Taisuke;SAKAGUCHI Hideya |
| 分类号 |
C12N5/0793 |
主分类号 |
C12N5/0793 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of producing a cell aggregate comprising telencephalon or a partial tissue thereof, or an progenitor tissue thereof, comprising obtaining a telencephalon marker-positive aggregate by culturing an aggregate of pluripotent stem cells in suspension in the presence of a Wnt signal inhibitor and a TGFβ signal inhibitor, and further culturing the telencephalon marker-positive aggregate in suspension under a high oxygen partial pressure condition. |
| 地址 |
Wako-shi JP |
