Systems and methods for non-fasting LDL cholesterol assays

摘要

In one embodiment, a test strip for testing for cholesterol-related blood analytes in whole blood includes a red blood cell separation layer, the red blood cell separation layer separating red blood cells from a blood sample applied to the test strip as the blood sample flows downward through the red blood cell separation layer. The test strip further includes a reaction layer receiving the blood sample from the red blood cell separation layer, the reaction layer including POE-POP-POE block copolymer, a surfactant, and a reflectivity changing reactant, the POE-POP-POE block copolymers solubilizing essentially only non-LDL cholesterol analytes, the non-LDL cholesterol analytes reacting with the reflectivity changing reactant in order to change a reflectivity of the blood sample.

主权项

1. A method of measuring LDL cholesterol from a human subject providing a blood sample, the blood sample including chylomicrons, the method comprising:
providing a dry test strip; receiving the blood sample at the dry test strip; separating red blood cells from the blood sample in a first layer of the dry test strip; reacting all non-LD cholesterol including chylomicrons in the blood sample in a reaction layer; producing a color change proportional to the non-LDL cholesterol; measuring the color change to determine a non-LDL cholesterol amount in the blood sample; and subtracting the non-LDL cholesterol amount from a total cholesterol amount in the blood sample to yield an LDL cholesterol amount for the blood sample wherein specificity of the reacting non-LDL cholesterol in the blood sample, due to a chemistry that solubilizes all cholesterols, including chylomicrons, other than LDL in the reaction layer, is such that accuracy is not substantially negatively affected by the presence of chylomicrons.