| 主权项 |
1. A peptide substrate selectively recognized by BoNT/E comprising the sequence(SEQ ID NO: 3)X1X2X3X4X5LX6GX7EIDTX8NRQX9DX10IX11X12KAX13X14X15X16X17, wherein: X1, X2 or X3: W, 1-Nal (1-Naphthylalanine), 2-Nal, F, Y, I, L, Nle (norleucine), A, or any other hydrophobic amino acid residue; X4: A or G; X5: N, K, A, or G; X6: No residue, or RHMALDM; X7: Nor Q; X8: Q, R, K or any other positively charged residue; X9: I, F, K, or R; X10: R, homoArg, Cit (citrulline) or Om (ornithine); X11: M or Nle; X12: A, F, G, I; X13: D, K, R or any other positively charged residue; X14: S, K, R or any other positively charged residue; X15: N, K, R or any other positively charged residue; X16: K, R or any other positively charged residue; and X17: R, TR; native or amidated C-terminus. |
