| 摘要 |
<p>PURPOSE:To enable a reliable diagnosis and curing of non-A, non-B hepatitis, by using a non-A, non-B hepatitis related antigen separated and refined from an autopsy liver of a non-A, non-B hepatitis patient, or a conjugate of said antigen with a sheep erythrocyte, an isotope, an enzyme or the like as a diagnostic. CONSTITUTION:A non-A, non-B hepatitis related antigen, which has MW of 1,500,000 or more (by a gel filtration method), a sedimentation constant (10<-12>) of 51.5S (by an ultracentrifugal analysis), a buoyant density (g/cm<2>) of 1.15- 1.25 (in cesium chloride and in KBr), a particle diameter (nm) of 26-37, and an electrophoretic mobility in an alpha2-alpha1 globulin region in agarose gel, is separated and refined from an autopsy liver of a non-A, non-B hepatitis patient by a specified treatment. A conjugate of the obtained antigen with a minute particle such as sheep erythrocyte, an isotope such as <125>I, alkali phosphathase or the like is prepared to obtain a diagnostic for immunological analysis. Thus a diagnostic which clearly distinguishes non-A, non-B hepatitis from other hepatitis.</p> |
