GENETICALLY ENGINEERED MICROORGANISMS FOR THE PRODUCTION OF CHORISMATE-DERIVED PRODUCTS

摘要

The invention provides genetically engineered microorganisms and methods for producing chorismate-derived products, such as para-hydroxybenzoic acid, salicylate, 2-aminobenzoate, 2,3-dihydroxybenzoate, and 4-hydroxycyclohexane carboxylic acid. Typically, the microorganism comprises at least one of (a) an exogenous chorismate pyruvate lyase, (b) an exogenous isochorismate synthase, (c) an exogenous isochorismate pyruvate lyase, and (d) a prephenate synthase comprising a disruptive mutation.

主权项

1. A genetically engineered C1-fixing bacterium capable of producing at least one chorismate-derived product, wherein the bacterium comprises at least one of:
a. an exogenous chorismate pyruvate lyase (EC 4.1.3.40), b. an exogenous isochorismate synthase (EC 5.4.4.2), c. an exogenous isochorismate pyruvate lyase (EC 4.2.99.21), and d. a prephenate synthase (EC 5.4.99.5) comprising a disruptive mutation.