CHROMATIN IMMUNOCAPTURE DEVICES AND METHODS OF USE

摘要

This application provides fluidic devices, such as microfluidic devices, which can be used for the creation and/or manipulation of droplets in droplet-based microfluidic systems, as well as systems and methods for using the same. The microfluidic devices can be used to generate droplets, extract or inject volume to droplets, and/or split droplets. Also provided are methods for generating nucleosomes, and isolated DNA from nucleosomes (or from non-nucleosomes), for example using the disclosed devices.

主权项

1. An automated method of generating nucleosomes, comprising:
encapsulating an aqueous solution comprising a single cell and a lysing solution within a droplet, wherein the single cell comprises nucleosomes, and wherein the droplet comprises oil and a surfactant, thereby lysing the cell within the droplet; adding to the droplet a non-nucleosome-cleaving nuclease solution, thereby cleaving DNA in the droplet without substantially affecting portions of the DNA that interact with histones within the nucleosomes; and adding a plurality of the droplets to an aqueous quenching solution, thereby quenching the non-nucleosome-cleaving nuclease and generating a population of nucleosomes in an aqueous phase.