Kits and methods for reprograming non-hepatocyte cells into hepatocyte cells
摘要
A method for inducing reprograming of a cell of a first type which is not a non-hepatocyte (non-hepatocyte cell), into a cell with functional hepatic drug metabolizing and transporting capabilities, is disclosed. The non-hepatocyte is induced to express or overexpress hepatic fate conversion and maturation factors, cultured in somatic cell culture medium, hepatocyte cell culture medium and hepatocyte maturation medium for a sufficient period of time to convert the non-hepatocyte cell into a cell with hepatocyte-like properties. The iHeps induced according to the methods disclosed herein are functional induced hepatocytes (iHeps) in that they express I and II drug-metabolizing enzymes and phase III drug transporters and show superior drug metabolizing activity compared to iHeps obtained by prior art methods. The iHeps thus provide a cell resource for pharmaceutical applications.
申请公布号
AU2015218082(A1)
申请公布日期
2016.09.29
申请号
AU20150218082
申请日期
2015.02.04
申请人
Peking University;Stem Cell and Regenerative Medicine Translational Research Institute;Beijing Vitalstar Biotechnology Co., Ltd.
发明人
Deng, Hongkui;Du, Yuanyuan;Shi, Yan;Jia, Jun;Wang, Jinlin;Xiang, Chengang;Song, Nan;Xu, Jun;Yin, Ming