| 摘要 |
PURPOSE:To detect the amount of amylase in a sample by a method wherein glucose present in the sample is converted to another substance, the processing solution used for such conversion is made contact with an enzyme electrode, and an amount of maltose produced under the action of amylase is measured. CONSTITUTION:Air bubbles generated from an air bubble generator 23 are charged into a buffer solution containing substrates from a tank 21 to separate the buffer solution into a number of segments each having a constant volume along a flow passage system 25 leading from a pump 22. When a sample is then injected to the solution from a sample injector 24, the enzyme reaction is started and a part of the substrates begins to decompose into maltose under the action of amylase present in the sample. Subsequently, the flow solution passes through a fixed enzyme section 26 inclusive of fixed enzymes having glucose as substrates. The glucose present in the flow solution is herein converted to a substance inactive to detection. Next, the flow solution enters a reaction section 27 where a production amount of maltose to be detected is increased, and the amount of maltose is then measured by a detector 29. |
