Biomarkers of osteoarthritis

摘要

Biomarkers, biomarker panels and methods for diagnosing osteoarthritis (OA) and determining treatment are disclosed, using measurement of the expression level of certain polypeptides in a test sample from a subject, including MCP1, IL8, KC, MMP2, MMP3, Apolipoprotein A1, and Apolipoprotein E. Related methods for monitoring OA treatment efficacy, diagnostic reagents, and kits are also described.

主权项

1. A method to detect osteoarthritis in a canine subject comprising:
(a) using a protein detection method to determine the levels of three or more different osteoarthritis marker polypeptides in a synovial fluid sample collected from a first canine subject, wherein the three or more different osteoarthritis marker polypeptides comprise MCP1, IL8, and KC; (b) using the protein detection method to determine the levels of three or more different osteoarthritis marker polypeptides in a biological fluid sample collected from a second canine control subject, wherein the three or more different osteoarthritis marker polypeptides comprise MCP1, IL8, and KC; (c) using the level of each of the three or more different osteoarthritis marker polypeptides in the first canine subject and the second canine control subject to calculate a probability of the presence of osteoarthritis in the first canine subject, and determining whether the probability is greater than or equal to at least about 80%; and (d) when the probability from step (c) is greater than or equal to at least about 80%, determining that osteoarthritis is present in the first canine subject.