| 主权项 |
1. A cuvette comprising a sample chamber configured to hold a sample, at least a portion of said cuvette comprising an optically transmissive material, said optically transmissive material comprising an optically transmissive surface and a reflective surface, wherein said optically transmissive surface and said reflective surface are shaped and positioned to simultaneously provide a plurality of light paths from light provided by a single external light source, said plurality of light paths comprising:
i) a direct light path through said optically transmissive surface and to said sample chamber, and ii) an indirect light path through said optically transmissive surface and within said optically transmissive material to said sample chamber, said indirect light path comprising a point of internal reflection at a reflective surface of said cuvette, effective that light from said external light source passing through the optically transmissive surface simultaneously provides both epi-illumination and trans-illumination to said sample in the sample chamber, where epi-illumination comprises light traveling through said optically transmissive surface and further along a direct light path to the sample without reflection at a surface of the optically transmissive material, and where trans-illumination comprises light traveling through said optically transmissive surface and further along an indirect light path within the optically transmissive material and to the sample comprising at least one point of internal reflection from at least one reflective surface of said optically transmissive material. |
