Methods and systems for microfluidic DNA sample preparation

摘要

The present invention relates to methods and systems for microfluidic DNA sample preparation. More specifically, embodiments of the present invention relate to methods and systems for the isolation of DNA from patient samples on a microfluidic device and use of the DNA for downstream processing, such as performing amplification reactions and thermal melt analysis on the microfluidic device.

主权项

1. A method of purifying DNA in a sample in a microfluidic device comprising the steps of:
(a) mixing the sample and a lysis buffer in a mixing region of a microfluidic device; (b) selectively lysing the cellular membranes of cells in the sample without lysing the nuclear membranes of cells in a cell lysing region of the microfluidic device to produce intact nuclei from the cells; (c) flowing a sample flow containing the intact nuclei into a cell trapping region and trapping the intact nuclei using a filter in the cell trapping region of the microfluidic device while flowing a cross-flow buffer and other components of the sample across the sample flow and through the filter into a waste collection region of the microfluidic device; (d) lysing the intact nuclei trapped by the filter; (e) releasing the DNA from the lysed nuclei; and (f) collecting the released DNA in a DNA collection region of the microfluidic device.