FREMGANGSMAATE FOR AA TRANSFORMERE SPOROGEN BACILLUS SUBTILIS RUB 830 TIL ASPOROGEN BACILLUS SUBTILIS RUB 331 SOM ER ANVENDELIG SOM EN VERT I ET VERT-VEKTOR SYSTEM.

摘要

Asporogenous mutant B. subtilis RUB 331 (ATCC 31578) and a process for using such mutant are disclosed. The asporogenous mutant desirably reverts to sporeformers with a frequency not greater than 10-7 reversions per bacterium per generation and meets the requirements of the NIH "Guidelines for Research Involving Recombinant DNA Molecules" for a B. subtilis host component of a Host-Vector 1 system.