| 主权项 |
1. A synthetic site-specific RNA endonuclease which comprises:
(i) an RNA binding protein domain that comprises a variant of the human Pumilio 1 homology domain, wherein said variant comprises eight 36-mer repeats, wherein each of the eight 36-mer repeats binds to a single nucleotide in an eight-nucleotide target RNA, wherein said eight-nucleotide target RNA comprises at least one cytosine, and wherein said variant comprises all of amino acids 828 to 1176 of SEQ ID NO:149 except for modifications at one or more positions corresponding to:
(a) positions 863 to 867 of SEQ ID NO:149,(b) positions 899 to 903 of SEQ ID NO:149,(c) positions 935 to 939 of SEQ ID NO:149,(d) positions 971 to 975 of SEQ ID NO:149,(e) positions 1007 to 1011 of SEQ ID NO:149,(f) positions 1043 to 1047 of SEQ ID NO:149,(g) positions 1079 to 1083 of SEQ ID NO:149, and/or(h) positions 1122 to 1126 of SEQ ID NO:149,wherein said modifications result in the variant comprising, in any combination, SerXXXGlu (SEQ ID NO:1) to bind guanine, (Cys/Ser)XXXGln (SEQ ID NO:66) to bind adenine, AsnXXXGln (SEQ ID NO:3) to bind uracil, and/or SerTyrXXArg (SEQ ID NO:4) to bind cytosine, wherein X is any amino acid;
(ii) a linker peptide; and (iii) a cleavage domain that comprises a PilT N-terminus (PIN) domain of human SMG6, wherein the RNA binding domain is at the amino terminus of the synthetic site-specific RNA endonuclease and the cleavage domain is at the carboxy terminus of the synthetic site-specific RNA endonuclease and wherein the cleavage domain cleaves u s stream and/or downstream of the eight-nucleotide target RNA. |
