Molecular probe for sphingolipids

摘要

There is presently provided a probe comprising an isolated sphingolipid binding domain (SBD) polypeptide, wherein the isolated SBD polypeptide is capable of binding to a sphingolipid, and methods and uses relating to such a probe.

主权项

1. A probe comprising:
an isolated sphingolipid binding domain (SBD) polypeptide consisting of the sequence set forth in SEQ ID NO.: 1 and being capable of binding to a sphingolipid; and a moiety that is to be targeted to a sphingolipid and that is:
(i) a detectable label detectable within a living cell in a non-invasive manner which is selected from the group consisting of a fluorescent group, a chemiluminescent group, a radioactive group, a photolabile fluorescent group, a protein cross-linker, a paramagnetic group and a heavy metal complex; or(ii) an antibody;the moiety being coupled to the SBD polypeptide via cysteine-[amino-ethoxy-ethoxy-acetyl]2 or [amino-ethoxy-ethoxy-acetyl]2.