LATEXREAGENS, SOM INNEHALLER LATEXPARTIKLAR SENSIBILISERADE MED ETT STEROID-SERUMALBUMINKONJUGAT, FOR IMMUNKEMISK BESTEMNING AV STEROID I MENSKLIG KROPPSVETSKA, SAMT FORFARANDE FOR FRAMSTELLNING AV REAGENSET

摘要

Latex reagents for quantitative assay of a variety of steroid hormones or metabolites thereof which are contained in body fluid or excreted fluid of human beings comprise latex particles which are immunologically sensitized with a conjugate selected from a variety of steroid-serum albumin conjugates in which the steroid to be detected is bonded to serum albumin in a very small ratio ranging from 0.5 to 7 molecules per 1 molecule of the serum albumin used. Sensitization of latex particles with such a conjugate is performed using a limited amount dependent on the bonding ratio of steroid molecules per molecule of serum albumin in the conjugate and the particle size of the latex used. The latex reagents show very high sensitivity amounting to 0.2-0.04 nmole steroid equivalent/ml.