| 摘要 |
PURPOSE:To collect carbonylreductase capable of reducing carbonyl groups in the presence of reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), by cultivating a microorganism, belonging to the genus Mucor, etc., and having the ability to produce the titled substance capable of giving an alcohol of S-configuration. CONSTITUTION:A microorganism, belonging to the genus Mucor or Phizopus, e.g. Mucor rouxianus or Rhizopus oryzae, and having the ability to produce carbonylreductase is cultivated in a nurient culture medium by the spinner culture method with aeration to give microbial cells from the resultant culture fluid, and the carboylreductase is extracted from the interior of the microbial cells. The resultant carbonylreductase is capable of reducing carbonyl groups which are constituent units of conjugated polyketones in the presence of reduced form of nicotinamide dinucleotide phosphate (NADPH) to produce 1mol each alcohol of S-configuration and nicotinamide adenine dinucleotide phosphate (NADP<+>) from 1mol each carbonyl groups and NADPH. The optimum pH is 6-7, and the molecular weight measured by the gel filtration method is 54,000+ or -2,000. |
