| 发明名称 |
METHOD OF PRINTING A TISSUE CONSTRUCT WITH EMBEDDED VASCULATURE |
| 摘要 |
A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition. The fugitive ink is then removed to create vascular channels in the extracellular matrix composition, thereby forming an interpenetrating vascular network in a tissue construct. |
| 申请公布号 |
US2016287756(A1) |
申请公布日期 |
2016.10.06 |
| 申请号 |
US201415146613 |
申请日期 |
2014.11.04 |
| 申请人 |
President and Fellows of Harvard College |
发明人 |
Lewis Jennifer A.;Kolesky David B.;Skylar-Scott Mark A.;Homan Kimberly A.;Truby Ryan L.;Gladman Amelia Sydney |
| 分类号 |
A61L27/50;A61L27/22;A61F2/06;B29C67/00;B33Y10/00;B33Y80/00;A61L27/38;A61L27/36 |
主分类号 |
A61L27/50 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of printing a tissue construct with embedded vasculature, the method comprising:
depositing one or more cell-laden filaments each comprising a plurality of viable cells on a substrate to form one or more tissue patterns, each of the tissue patterns comprising one or more predetermined cell types; depositing one or more sacrificial filaments on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns, each of the sacrificial filaments comprising a fugitive ink; at least partially surrounding the one or more tissue patterns and the vascular pattern with an extracellular matrix composition, removing the fugitive ink to create vascular channels in the extracellular matrix composition, thereby forming an interpenetrating vascular network in a tissue construct. |
| 地址 |
Cambridge MA US |
