| 摘要 |
PURPOSE:To obtain ophthalmic acid useful as a raw material for eyewash by one process simply and efficiently, by bringing L-glutamic acid, L-alpha- aminobutyric acid and glycine into contact with specific Escherichia coli in the presence of acetyl phosphate. CONSTITUTION:Escherichia coli having activity of gamma-glutamylcysteine glutathione synthetase and acetate kinase or a treated material thereof is brought into contact with an aqueous solution containing L-glutamic acid. L-alpha-aminobutyric acid, glycine and adenosine triphosphate in the presence of acetyl phosphate to produce gamma-L-glutamyl-L-alpha-amino-n-butyrylglycine(ophthalmic acid) by one process. Escherichia coli having enlarged gamma-glutamylcysteine synthetase and glutathione synthetase enlarged by gene recombination is preferable as the Escherichia coli used. |
