发明名称 |
NOVEL PLASMID, PRODUCTION THEREOF AND NOVEL BACTERIUM CONTAINING SAME |
摘要 |
PURPOSE:To produce a useful transformed bacterium, by forming a plasmid integrated with a deoxyribonucleic acid fragment carrying genetic information participating in secretory production of alkali CGTase of a bacterium belonging to the genus Bacillus at a restriction site. CONSTITUTION:A DNA fragment providing secretory production ability of alkali cyclomaltodextrin glucanotransferase (CGTase for short) of a bacterium belonging to the genus Bacillus is prepared by using a restriction enzyme. Vector DNA of plasmid is prepared by using a restriction enzyme which does not inhibit genetic information participating in secretory production of alkali CGTase of the DNA fragment. Further, the restriction site of the vector DNA of plasmid is removed with the above-mentioned DNA fragment and the recombinant plasmid is extracted to give a plasmid. |
申请公布号 |
JPS62208287(A) |
申请公布日期 |
1987.09.12 |
申请号 |
JP19860050973 |
申请日期 |
1986.03.08 |
申请人 |
OJI KOONSUTAAC KK |
发明人 |
YAMANE KUNIO;KIMURA KENJI |
分类号 |
C12N15/09;C12N1/20;C12N1/21;C12N9/10;C12N15/00;C12R1/07;C12R1/125;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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