Strain mass-producing Epsilon-poly-L-lysine, a method for using its strain and a method for producing Epsilon-poly-L-lysine.

摘要

The present invention provides variant strains mass-producing epsilon PL which are obtained by variant treatment of a strain producing epsilon PL. For using the variant strains, the strains are cultured in a cultivated medium, epsilon PL is mass-produced and stored in the culture solution, and the stored epsilon PL is collected from the solution. For producing epsilon PL, a strain which produces epsilon PL is variant-treated, the obtained variant strain is cultivated in a culture medium to which L-lysine or L-lysine and one or more sugars are added, epsilon PL is mass-produced and stored in the culture solution, and the stored epsilon PL is collected from the solution. The variant strains producing epsilon PL in large quantities are preferably the strains which have tolerance to an analogue of L-lysine of Streptomyces albulus subsp. lysinopolymerus No.346-D strain and plasmid-amplifiable variant strains of the same bacteria.