Controlled release of periplasmic proteins into the medium.

摘要

This invention relates to methods and intermediates which cause the release of periplasmic proteins of gram-negative bacteria into the growth medium. This invention relates more particularly to novel DNA transfer vectors and gram-negative hosts transformed with them. These vectors are characterized in that they include a DNA sequence encoding a protein, such as kil, which when expressed causes proteins located in the periplasmic space of gram-negative bacteria to be released into the culture medium. It also relates to vectors, transformed hosts and methods for the controlled expression of the kil gene product.